Vibrio cholerae is a gram negative bacteria which is rod shaped and has a distinct flagellum. It is a facultative anaerobe which means it can survive with or without oxygen. It is highly infectious and causes cholera which symptoms are diarrhoea, vomiting and stomach cramps.
more on cholera:
http://www.nhs.uk/Conditions/Cholera/Pages/Definition.aspx_
more on cholera:
http://www.nhs.uk/Conditions/Cholera/Pages/Definition.aspx_
Thiosulphate citrate bile salts sucrose sugar (TCBS)
Vibrio cholerae is shown as flat yellow colonies on TCBS agar plates meaning they stand out and are easier to isolate.
- A selective medium used for the isolation of vibrio cholerae.
- Sodium biosulphate provides a high pH which inhibits the growth of gram positive bacteria.
- Bile salts also inhibit the growth of gram positive bacteria by combining with phospholipids to break down fat globules in the process of emulsification by associating its hydrophobic side with lipids and the hydrophilic side with water.
- Sodium chloride also provides an alkaline environment which provides the optimum growth of vibrio bacterium.
Vibrio cholerae is shown as flat yellow colonies on TCBS agar plates meaning they stand out and are easier to isolate.