Method:
1. Wash hands and sterilize work area using ethanol to kill any bacteria on the surface.
2. Light the bunsen burner, this creates an updraft to prevent bacteria landing on the agar or inoculation loop.
3. Move the inoculation loop in the hottest part of the flame until it terns red hot to sterilize it.
4. Remove the bung from the test tube containing the monoculture and flame the neck. (without putting the bung down to prevent contamination)
5. Dip the inoculation loop into the monoculture and flame the neck of the test tube again before replacing the bung.
6. Open the petri dish containing the nutrient agar near the bunsen burner and run the inoculation loop over the agar turning it 90 degrees each time.
7. Replace the lid of the petri dish and flame the inoculation loop again.
8. Tape 4 corners of the petri dish to make sure the lid stays on and also to allow oxygen to enter.
1. Wash hands and sterilize work area using ethanol to kill any bacteria on the surface.
2. Light the bunsen burner, this creates an updraft to prevent bacteria landing on the agar or inoculation loop.
3. Move the inoculation loop in the hottest part of the flame until it terns red hot to sterilize it.
4. Remove the bung from the test tube containing the monoculture and flame the neck. (without putting the bung down to prevent contamination)
5. Dip the inoculation loop into the monoculture and flame the neck of the test tube again before replacing the bung.
6. Open the petri dish containing the nutrient agar near the bunsen burner and run the inoculation loop over the agar turning it 90 degrees each time.
7. Replace the lid of the petri dish and flame the inoculation loop again.
8. Tape 4 corners of the petri dish to make sure the lid stays on and also to allow oxygen to enter.